Citrons have been widely used for medicinal purposes for a long time, but the application of\ncitron in the food industry is still restricted. The extensive advantages of nanotechnology in the food\nindustry have greatly broadened the application of foods. In this study, by employing nanotechnology,\nwe prepared citron-extract nanoparticle with an average size of 174.11 �± 3.89 nm, containing protein\npeptide and/or liposome. In order to evaluate the toxicity of nanoparticles and to ensure food safety,\nbiological cytotoxicity at the cell and genomic levels was also identified to examine the toxicity\nof citron extracts by using an in vitro system. Our results demonstrated that the cytotoxicity of\ncitronliposome was dependent on cell type in high concentrations (1 and 5 mg/mL), selectively against\nprimary human cardiac progenitor cells (hCPCs), and human endothelial progenitor cells (hEPCs) in\nMTT and lactate dehydrogenase (LDH) assays. Interestingly, for the NIH-3T3 and H9C2 cell lines,\ncell cytotoxicity was observed with slight genotoxicity, especially from citronpeptide extract for both\ncell lines. Taken together, our study provides cytotoxicity data on nanoengineered citron extracts\naccording to different cell type as is crucial for further applications.
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